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lithium9738新蟲 (初入文壇)
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[求助]
實(shí)驗(yàn)性肺轉(zhuǎn)移模型注射癌細(xì)胞前用制劑預(yù)孵育的原因
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最近看到一篇文獻(xiàn)中:Antimetastatic Activity in Experimental Lung Metastasis Model. Five-week old female Balb/c mice were assigned into 14 groups (n = 5). Five ×105 4T1 cells were pretreated with AMD3100(1 mM), and polyplexes (w/w 4) for 4 h before i.v. injection in 100μL PBS (Day 1). The animals were then treated i.v. with AMD3100(1.25 mg/kg) and polyplexes (1.2 mg/kg siScr) on day 3, 5, 7, and 9 and sacrificed on day 11. 請問這里為什么在尾靜脈注射4T1細(xì)胞建立肺轉(zhuǎn)移模型之前用制劑進(jìn)行預(yù)孵育,有什么好處,求助! |
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