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機(jī)械蟲996新蟲 (正式寫手)
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[求助]
納米級的纖維素晶體懸浮液
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納米級的纖維素懸浮液怎么才能得到纖維素? 離心加透析? 透析需要哪些儀器耗材,怎么操作? @流浪南昌 @初次見面 @bionics 發(fā)自小木蟲Android客戶端 |
金蟲 (正式寫手)
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你說的后面的那個(gè)纖維素,是指單個(gè)纖維素分子嗎?透析用透析袋就行,可以有不同分子量的,用于蛋白的純化,一般是小分子出去,蛋白留袋子里。透纖維素我沒見過,如果你有看到相關(guān)文獻(xiàn),可以分享分享 發(fā)自小木蟲IOS客戶端 |
新蟲 (正式寫手)
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The linter was ground in a Wiley mill and hydrolyzed without any chemical pretreatment. The method of acidic hydrolysis was applied with minor adaptations. The linter was mechanically stirred at a ratio of 1:20 (w/v) of aqueous concentrated sulfuric acid (60%, w/w) with a Teflon?bar dispersing element, at 45?°C, for 60?min. The nanowhiskers suspension was centrifuged for 15?min at 13,000?rpm in a High-speed Refrigerated Centrifuge CR22GIII, and the precipitate was resuspended in distilled water and dialyzed with tap water until a pH (6–7) was reached. The process from centrifugation through dialysis was repeated three times. 棉絨在Wiley磨機(jī)中研磨,并在沒有任何化學(xué)預(yù)處理的情況下水解。酸性水解方法(Cranston和Gray,2006,Medeiros等人,2008,Orts等人,2005)經(jīng)過輕微調(diào)整。 將棉絨以1:20(w / v)與特氟龍棒分散元件的比例在60°C下機(jī)械攪拌45分鐘。將納米晶須懸浮液在高速冷凍離心機(jī)CR60GIII中以13000rpm離心15分鐘,并將沉淀物重懸于蒸餾水中并用自來水透析直至達(dá)到pH(6-7)。從離心到透析的過程重復(fù)三次。 發(fā)自小木蟲Android客戶端 |
新蟲 (正式寫手)
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文獻(xiàn)名稱:Extraction and characterization of nanocellulose structures from raw cotton linter 發(fā)自小木蟲Android客戶端 |
新蟲 (正式寫手)
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