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[求助]
求大牛指教 海藻酸鈉 3D培養(yǎng) 已有3人參與
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求大牛指教,F(xiàn)在使用電紡儀制備微球(電噴霧法),希望細胞能在微球上生長,使用的材料是海藻酸鈉,但是細胞基本不會貼在微球上。我嘗試過在微球的表面交聯(lián)一層明膠,但是也不怎么有改善。希望大牛指教!![]() ![]() |
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嘗試在微球的表面交聯(lián)一層polyArg-Lys 或直接參考 10 branched poly(Arg, epsilon-Lys)-hyaluronic acid hydrogel (for 3D cell culture) Xenogenic-free, chemically defined, highly reproducible and excellent biocompatibility Without crosslinker and organic solution Mimic the natural extracellular matrix(ECM). Proper mechanical strength Storage at room temperature No need to pre-treat prior to using with cell suspension To perform assays on isolated spheroids, dissolve PHA with iso-osmolar trisodium citrate. Mesh size 50, 100, 150μm |
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